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Status |
Public on May 20, 2015 |
Title |
Drop-Seq analysis of mixtures of human (HEK) and mouse (3T3) cells (1000/100 STAMPs) at 50 cells per microliter |
Organisms |
Homo sapiens; Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing Other
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Summary |
A cell supsension containing an equal mix of HEK and 3T3 cells was used in Drop-Seq, a novel technology for high-throughput single cell mRNAseq. The cells were mixed at a final concentration of 50 cells per microliter.
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Overall design |
An estimated 1000 STAMPs and 100 STAMPs were separately selected for sequencing to obtain both high coverage and broad coverage data
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Contributor(s) |
Macosko EZ |
Citation(s) |
26000488, 32888429 |
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Submission date |
Nov 13, 2014 |
Last update date |
Apr 25, 2022 |
Contact name |
Evan Macosko |
E-mail(s) |
emacosko@genetics.med.harvard.edu
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Organization name |
Harvard Medical School
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Department |
Genetics
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Lab |
McCarroll Lab
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Street address |
77 Avenue Louis Pasteur, NRB 260
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
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Platforms (1) |
GPL19415 |
Illumina NextSeq 500 (Homo sapiens; Mus musculus) |
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Samples (2) |
GSM1544798 |
SpeciesMix_ThousandSTAMPs_50cellspermicroliter |
GSM1544799 |
SpeciesMix_HundredSTAMPs_50cellpermicroliter |
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This SubSeries is part of SuperSeries: |
GSE63473 |
Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets |
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Relations |
BioProject |
PRJNA267124 |
SRA |
SRP049775 |