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Series GSE63166 Query DataSets for GSE63166
Status Public on May 06, 2015
Title Small RNA and transcriptome profiling of mice deficient in either germline Dgcr8 or Dicer
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary We used Illumina Small RNA and RNA-Seq kits to prepare both small RNA and RNA-Seq libraries from total RNA isolated from either leptotenze/zygotene or pachytene spermatocytes purified from either Dgcr8 or Dicer germline conditional knockout mice.
Overall design Conditional knockout mice were generated by using a Ddx4 promoter to drive cre excision of either Dgcr8 or Dicer at embryonic day 18. Mixed leptotene/zygotene or pachytene spermatocytes were then isolated from the testis of adult conditional knockout mice, along with paired WT littermates as a control. RNA was isolated from these spermatocytes using Trizol. Small RNA or RNA-Seq libraries were then prepped using Illumina's sequencing library preparation kits.
Contributor(s) Hilz S, Modzelewski A, Fogarty E, Grimson A, Cohen P
Citation(s) 25934699
Submission date Nov 10, 2014
Last update date May 15, 2019
Contact name Andrew Grimson
Organization name Cornell University
Department Molecular Biology & Genetics
Street address 445 Biotechnology Building
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (16)
GSM1542508 Dgcr8 WT L/Z SMALL RNA
GSM1542509 Dgcr8 cKO L/Z SMALL RNA
GSM1542510 Dicer WT L/Z SMALL RNA
BioProject PRJNA266844
SRA SRP049679

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Supplementary file Size Download File type/resource
GSE63166_DgLWvK_edgeR_results_20141106_wCPMs.csv.gz 496.3 Kb (ftp)(http) CSV
GSE63166_DgPWvK_edgeR_results_20141106_wCPMs.csv.gz 516.3 Kb (ftp)(http) CSV
GSE63166_DiLWvK_edgeR_results_20141106_wCPMs.csv.gz 483.6 Kb (ftp)(http) CSV
GSE63166_DiPWvK_edgeR_results_20141106_wCPMs.csv.gz 527.5 Kb (ftp)(http) CSV
GSE63166_miRNA_Analysis.txt.gz 20.3 Kb (ftp)(http) TXT
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