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Series GSE63011 Query DataSets for GSE63011
Status Public on Nov 02, 2015
Title Heterochromatin remodeling by CDK12 contributes to learning in Drosophila
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We describe the epigenetic profiling of the H3K9me2 and HP1a in Drosophila third instar larvae before and after CDK12 depletion by RNA interference (RNAi). Here we show that CDK12 regulates heterochromatin dynamics in Drosophila chromosomes. Depletion of CDK12 induces the increased HP1a and H3K9me2 binding profile on the coding region of euchromatic genes, with the X chromosome being the most affected. These results are consistent with the polytene chromosome immunostaining pattern of HP1a and H3K9me2 after CDK12 knockdown in our initial cytological observations, which show that CDK12 depletion induce heterochromatin spreading on euchromatic arms, especially on the X chromosome. This study describes a novel role of the CDK12 complex in controlling the epigenetic transition between euchromatin and heterochromatin.
Overall design Examination of the genome-wide H3K9me2 and HP1a binding profile in wildtype larvae (WT) and CDK12-depleted larvae (CDK12-KD). Examination of the genome-wide CDK12 binding profile in wildtype larvae (WT). Twelve independent immunoprecipitations were conducted for each antibody. Two biological replicates were performed.
Contributor(s) Pan LX, Xie W, Li KL, Yang Z, Xu J, Zhang W, Liu LP, Ren X, He Z, Wu J, Sun J, Wei HM, Wang DL, Xie W, Li W, Ni JQ, Sun FL
Citation(s) 26508632
Submission date Nov 05, 2014
Last update date May 15, 2019
Contact name Li xia Pan
Organization name Tsinghua university
Department School of medicine
Lab C304
Street address North Zhongguancun Street
City Beijing
State/province Beijing
ZIP/Postal code 100084
Country China
Platforms (2)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
GPL19951 Illumina HiSeq 1500 (Drosophila melanogaster)
Samples (16)
GSM1537957 Drosophila_larvae_WT_H3K9me2_ChIP-Seq_rep1
GSM1537958 Drosophila_larvae_CDK12-KD_H3K9me2_ChIP-Seq_rep1
GSM1537959 Drosophila_larvae_WT_HP1a_ChIP-Seq_rep1
BioProject PRJNA266433
SRA SRP049554

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Supplementary file Size Download File type/resource
GSE63011_RAW.tar 556.6 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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