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Series GSE62936 Query DataSets for GSE62936
Status Public on Dec 30, 2014
Title RNA-Seq Analysis in hES/ iPS cell-derived neuronal samples
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We characterized the gene expression by Hierarchical Clustering and one-matrix clustering in hESC, day 12 progenitors, day 25-day 27, day82 differentiated hypothalamic neurons from hESCs and day 45 neurons derived from iPSCs generated from controls (2 independent) and BBS (Bardet-Biedl Syndrome, 3 independent) subjects.
Overall design RNA was isolated from cells of 13 samples (1 hESC, triplicate for day 12 progenitors, 1 day 25 neuron sample, duplicate for day 27 neuron samples, 1 day 82 neuron sample, five day 45 neuron samples made from 5 independent iPSC lines ) using RNeasy Micro Kit (QIAGEN). Quality control of the RNA was carried out with the Agilent Bio-analyzer, Qubit 2.0 at the MPSR of Columbia University. 100 ng of RNA with RIN ≥ 9 were used for generating mRNA-focused libraries using TruSeq RNA Sample Preparation Kit v2 and sequencing on an Illumina 2000/2500 V3 Instrument offered by the Columbia Genome Center.
Contributor(s) Wang L, Egli D, Leibel RL
Citation(s) 25555215
Submission date Nov 03, 2014
Last update date May 15, 2019
Contact name Liheng Wang
Organization name Columbia University Medical Center
Department Department of Medicine
Lab Domenico Accili
Street address 1150 Saint Nicholas Avenue, Room 234
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (13)
GSM1536664 LW01
GSM1536665 LW02
GSM1536666 LW03
BioProject PRJNA266234
SRA SRP049475

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Supplementary file Size Download File type/resource
GSE62936_RAW.tar 11.9 Mb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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