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Series GSE62883 Query DataSets for GSE62883
Status Public on Oct 31, 2015
Title Expression profiling of small, noncoding RNAs in physiologically hypertrophied rat hearts
Organism Rattus norvegicus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Purpose: Different studies revealed the importance of microRNAs (miRNAs) in pathological hypertrophy but their role during physiological hypertrophy is largely unexplored. Hence, this study is aimed at revealing the global expression profile of miRNAs during physiological cardiac hypertrophy.
Methods: Chronic swimming protocol continuously for eight weeks resulted in induction of physiological hypertrophy in rats and histopathology revealed the absence of tissue damage, apoptosis or fibrosis. Subsequently, the total RNA was isolated and small RNA sequencing was executed in Illumina HiSeq 2000.
Results: Analysis of small RNA reads revealed the differential expression of a large set of miRNAs during physiological hypertrophy. The expression profile of the significantly differentially expressed miRNAs were validated by qPCR.
Conclusions: This is the first report to reveal the involvement of a large number of apoptotic miRNAs during physiological cardiac hypertrophy including the previously unknown cardiac players like miR-99, miR-100, miR-191, miR-181 and miR-19. Our data indicates that regulation of these apoptotic miRNAs can be one of the major key factor in determining pathological or physiological hypertrophy by controlling apoptosis, fibrosis and cell death mechanisms.
Overall design Heart small RNA profiles of 16-week old control and physiologically hypertrophied wistar rat were generated by deep sequencing using Illumina HiSeq 2000
Contributor(s) Ramasamy S, Velmurugan G
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Submission date Oct 31, 2014
Last update date May 15, 2019
Contact name Subbiah Ramasamy
Phone +914522458220
Organization name Madurai Kamaraj University
Department Molecular Biology
Lab Cardiac Hypertrophy Laboratory
Street address Palkalainagar
City Madurai
State/province Tamilnadu
ZIP/Postal code 625021
Country India
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (2)
GSM1535622 Sw_Con
GSM1535623 Sw_Hy
BioProject PRJNA265928
SRA SRP049429

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62883_RAW.tar 30.0 Kb (http)(custom) TAR (of XLSX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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