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Series GSE6272 Query DataSets for GSE6272
Status Public on Dec 31, 2006
Title House keeping gene analysis - carcinoids and normal mucosa
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Accurate and reproducible quantitation of target genes depends on correct normalization. Historically, genes with variable tissue transcription e.g. GAPDH, have been used as normalization factors which is problematic, particularly in clinical samples which often are derived from different tissue sources. Using a large-scale gene database (GeneChip (Affymetrix U133A) dataset of 36 gastrointestinal tumors and normal tissues), we identified 8 candidate reference genes that were highly expressed with low variability and established expression levels by real-time RT-PCR in an independent set of GI tissue samples (n=42).
Keywords: house-keeping, intestine, carcinoid
Overall design (GeneChip (Affymetrix U133A) dataset of 36 gastrointestinal tumors and Affymetrix protocol:
Contributor(s) Kidd MS, Modlin IM, Mane SM, Nadler B
Citation(s) 17456737
Submission date Nov 13, 2006
Last update date Aug 10, 2018
Contact name Mark Kidd
Phone 203 785-5429
Fax 203 737-4067
Organization name Yale University School of Medicine
Department Surgery
Lab Modlin Lab
Street address 333 Cedar Street
City New Haven
State/province CT
ZIP/Postal code 06525
Country USA
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (36)
GSM144265 Housekeeping gene analysis_S1
GSM144266 Housekeeping gene analysis_S2
GSM144267 Housekeeping gene analysis_S3
BioProject PRJNA99659

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