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Series GSE62668 Query DataSets for GSE62668
Status Public on Dec 04, 2014
Title Paternal diet defines offspring chromatin state and intergenerational obesity.
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary The global rise in obesity has revitalized a search to understand genetic, and in particular, epigenetic factors underlying the disease. We present a Drosophila model of paternal-diet-induced Inter-Generational Metabolic Reprogramming (IGMR) and identify genes required for its encoding in offspring. Intriguingly, we find that as little as two days of dietary intervention in fathers elicits obesity in offspring. Paternal sugar acts as a physiological suppressor of variegation, de-silencing chromatin state-defined transcriptional units in both mature sperm and in offspring embryos. We identify requirements for H3K9/K27me3 dependent reprogramming of metabolic genes in two distinct germline and zygotic windows. Critically, we find evidence that a similar system regulates obesity-susceptibility and phenotype variation in mice and humans. The findings provide insight into the mechanisms underlying intergenerational metabolic reprogramming and carry profound implications for our understanding of phenotypic variation and evolution.
Overall design RNA-seq on Drosophila embryos and sperm samples fed medium and high sugar.
Contributor(s) Öst A, Lempradl A, Casas E, Weigert M, Tiko T, Deniz M, Pantano L, Boenisch U, Itskov PM, Stoeckius M, Ruf M, Rajewsky N, Reuter G, Iovino N, Ribeiro C, Alenius M, Heyne S, Vavouri T, Pospisilik JA
Citation(s) 25480298
Submission date Oct 23, 2014
Last update date May 15, 2019
Contact name Tanya Vavouri
Organization name Josep Carreras Leukemia Research Institute
Street address Ctra de Can Ruti, Cami de les Escoles, s/n
City Badalona
State/province BARCELONA
ZIP/Postal code 08019
Country Spain
Platforms (1)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
Samples (8)
GSM1531190 F1 embryos of medium sugar fed male flies - Replicate 1
GSM1531191 F1 embryos of medium sugar fed male flies - Replicate 2
GSM1531192 F1 embryos of high sugar fed male flies - Replicate 1
BioProject PRJNA264746
SRA SRP049220

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Supplementary file Size Download File type/resource
GSE62668_Embryo.txt.gz 484.1 Kb (ftp)(http) TXT
GSE62668_Sperm.txt.gz 431.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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