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Series GSE62566 Query DataSets for GSE62566
Status Public on Nov 01, 2014
Title Characterization of the inflammatory phenotype of Mycobacterium avium subspecies paratuberculosis
Platform organisms Mycobacterium avium subsp. paratuberculosis; Mycobacterium avium subsp. avium
Sample organism Mycobacterium avium subsp. paratuberculosis
Experiment type Expression profiling by array
Summary Understanding the pathogenic mechanisms and host responses to Johne's disease, caused by Mycboacterium avium subspecies paratuberculosis (MAP), is complicated by a number of challenges of the disease progression and lack of ex vivo models to study the disease. We describe novel cell culture passage model which mimics the course of infection in vivo. Using this model we determined that cell culture passaged MAP develops an inflammatory phenotype initiating higher levels of pro-inflammatory cytokines and chemokines compared to levels initiated by non-passaged MAP. To verify that the MAP phenotypes were changing, we conducted transcriptome analysis of each population during the passage model and identified a change in gene expression, specifically and upregulation of genes involved in the biosynthesis of lipid components within the cell. Lipidomic analysis showed dramatically different lipid profiles between the 2 populations. These transcriptome changes were validated by the identification of high levels of upregulated transcripts from MAP-infected cattle ileal tissue. In total, this study described a novel model used to show that MAP changes phenotype during intracellular passage, thus changing its lipid profile and triggering an inflammatory response in the host leading to the severe clinical phage of Johne's disease.
 
Overall design In total 6 slides were analyzed. Of the 2 phenotypes under investigation, the P0 samples (non-inflammatory) were analyzed in triplate and the P2 samples (inflammatory) were analyzed in triplicate. Each slide fluorescence intensity was measured at PMT400, 600 and 750. Each oligo on each slide was spotted in triplicate and each slide had its own internal controls.
 
Contributor(s) Everman JL, Eckstein TM, Rousey J, Coussens P, Bannantine JP, Bermudez LE
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Submission date Oct 21, 2014
Last update date Nov 01, 2014
Contact name Luiz E Bermudez
Organization name Oregon State University
Department Biomedical Sciences
Street address 105 Dryden Hall
City Corvallis
State/province Oregon
ZIP/Postal code 97331
Country USA
 
Platforms (1)
GPL3433 NADC MAP Oligo v1.0
Samples (18)
GSM1529412 MAP_P0_PMT400_rep1
GSM1529413 MAP_P0_PMT600_rep1
GSM1529414 MAP_P0_PMT750_rep1
Relations
BioProject PRJNA264417

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62566_RAW.tar 19.6 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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