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| Status |
Public on Nov 09, 2006 |
| Title |
The effects of Serum Amyloid A on gene expression profile in HUVECs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
This study attemps to study the global effects of Serum Amyloid A (SAA) on human endothelial gene expression profile in different time points.
Keywords: time course
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| Overall design |
HUVECs were cultured to confluence in 6-well plates and stimulated with 20 μg/ml of SAA (PeproTech) for 0, 4, 24 and 48 h. To determine the cellular transcript profile in Control (0h) and 3 different SAA groups (S4h, S24h and S48h), the relative abundance of mRNAs in SAA groups were compared with Control by use of Human Genome U133 Plus 2.0 Array (HG-U133 Plus 2.0, Affymetrix)). Each group had 2 individual samples. . In order to obtain the 3 group comparison datasets (S4h/C, S24h/C and S48h/C), expression patterns for each individual SAA sample (4h, 24h and 48h) were compared to each individual control sample. Hence, each group comparison has 4 individual sample comparisons. The robust increased or decreased cut-off was use the Affymetrix recommending "1" or "-1" of the signal log ratio. . If the gene is robust “increased” or “decreased” in 4 individual sample comparisons of the total 4 comparisons, it was selected as the final robust changed in group comparison. Each gene we were interested, real time PCR was carried out to validate the microarray results.
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| Contributor(s) |
Zhao Y, Lye H, Sukardi H, Heng C |
| Citation(s) |
18552508 |
| Submission date |
Nov 07, 2006 |
| Last update date |
Mar 25, 2019 |
| Contact name |
yulan zhao |
| E-mail(s) |
g0203850@nus.edu.sg
|
| Phone |
(65)68746450
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| Fax |
(65)67742796
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| Organization name |
National University of Singapore
|
| Department |
Paediatrics
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| Lab |
Cardiovascular Genetics Lab
|
| Street address |
Level 1, Service Block, #B1-15
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| City |
Singapore |
| ZIP/Postal code |
119260 |
| Country |
Singapore |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA97511 |
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