|
| Status |
Public on Mar 01, 2007 |
| Title |
Histone turnover marks the boundaries of cis-regulatory domains |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Cellular memory is maintained at the Drosophila homeotic gene clusters by cis-regulatory elements who mechanism of action is unknown. We have examined chromatin dynamics in the Drosophila genome by measuring histone turnover levels at high resolution. Surprisingly, homeotic gene clusters display characteristic histone turnover profiles with conspicuous peaks at boundaries of cis-regulatory domains superimposed over regions of very low turnover. Peaks of histone turnover precisely correspond to nuclease hypersensitive sites for epigenetic silencing proteins. Our results suggest that epigenetic regulation is facilitated by histone turnover, which maintains continuous accessibility ov cis-regulatory DNA.
Keywords: Chromatin affinity-purification on microarray
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| |
|
| Overall design |
Chromatin affinity profiling of histones H3 and H3.3 on Drosophila tiling arrays
|
| |
|
| Contributor(s) |
Mito Y, Henikoff JG, Henikoff S |
| Citation(s) |
17347439 |
| |
| Submission date |
Nov 06, 2006 |
| Last update date |
Sep 20, 2012 |
| Contact name |
Jorja Henikoff |
| E-mail(s) |
jorja@fhcrc.org
|
| Phone |
206-667-4850
|
| Organization name |
Fred Hutchinson Cancer Research Center
|
| Department |
Basic Sciences
|
| Lab |
Henikoff
|
| Street address |
1100 Fairview AV N, A1-162
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-1024 |
| Country |
USA |
| |
|
| Platforms (4)
|
| GPL2678 |
Tiling design for D. melanogaster |
| GPL3352 |
FHCRC DMEL 4.2 Tiling 1 of 3 |
| GPL3353 |
FHCRC DMEL 4.2 Tiling 2 of 3 |
| GPL3354 |
FHCRC DMEL 4.2 Tiling 3 of 3 |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA97603 |
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