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Series GSE62305 Query DataSets for GSE62305
Status Public on May 11, 2015
Title CRISPR Display: A modular method for locus-specific targeting of long noncoding RNAs and synthetic RNA devices in vivo [RIP-Seq]
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Noncoding RNAs (ncRNAs) comprise an important class of natural regulators that mediate a vast array of biological processes, including the modulation of chromatin architecture. Moreover, artificial ncRNAs have revealed that the functional capabilities of RNA are extremely broad. To further investigate and harness these capabilities, we developed CRISPR-Display ("CRISP-Disp"), a targeted localization strategy that uses Cas9 to deploy large RNA cargos to specific DNA loci. We demonstrate that exogenous RNA domains can be functionally appended onto the CRISPR scaffold at multiple insertion points, allowing the construction of Cas9 complexes with RNAs nearing one kilobase in length, with structured RNAs, protein-binding cassettes, artificial aptamers and pools of random sequences. CRISP-Disp also allows the simultaneous multiplexing of disparate functions at multiple targets. We anticipate that this technology will provide a powerful method with which to ectopically localize functional RNAs and ribonuceloprotein complexes at specified genomic loci.
Overall design RNA Immunoprecipitation (RIP) against FLAG-tagged Cas9 protein, coexpressed with a large pool of CRISPR RNAs bearing random internal insertions
Contributor(s) Shechner DM, Younger ST, Hacisuleyman E, Rinn JL
Citation(s) 26030444
Submission date Oct 14, 2014
Last update date May 15, 2019
Contact name Scott T Younger
Organization name Children's Mercy Kansas City
Street address 2401 Gillham Road
City Kansas City
State/province MO
ZIP/Postal code 64108
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (7)
GSM1525041 Library
GSM1525042 Input_Replicate_1
GSM1525043 Input_Replicate_2
This SubSeries is part of SuperSeries:
GSE66756 CRISPR Display: A modular method for locus-specific targeting of long noncoding RNAs and synthetic RNA devices in vivo
BioProject PRJNA263806
SRA SRP048899

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Supplementary file Size Download File type/resource
GSE62305_Sequence_Counts.txt.gz 8.1 Mb (ftp)(http) TXT
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Processed data are available on Series record

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