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Series GSE62297 Query DataSets for GSE62297
Status Public on May 21, 2015
Title Whole-genome expression profiling of C. elegans larvae and adults
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary Temperature, as a universal enviromental factor, has prolonged effect on physiological and pathological functions of different species. In order to expolore the temperol effect of temperature on C.elegans longevity, we used microarray to check the whole-genome expression profiling of L4 larvae and Day3-old adults of C.elegans maintaining at different temperature
 
Overall design Adult worms are collected after maintained at 25 degree and 15 degree for 3days from later L4 stage, while Larva worms are collected after growing at 25 degree and 15 degree from embryo to late L4 stege. Then total RNA are extracted with TRI reagent(Life Technologies) from~120 adults or ~300 larvae. The concentrations and quality of total RNA are checked with Thermo Nanodrop 2000c(Thermo Scientific,WilminGton,DE) and Agilent 2100 BioAnalyzer (Agilent Technologies, Palo Alto, CA). Total RNA samples were hybrydized with Affymetrix C. elegans Gene 1.1 ST Array Strip. Preparation of cDNA, hybridization, quality controls and scanning of arrays were performed according to the manufacturer's protocol (Affymetrix, Santa Clara, CA) at the microarray core facility of University of Michigan .
 
Contributor(s) Xu XZ, Zhang B
Citation(s) 26027928
Submission date Oct 14, 2014
Last update date Aug 20, 2015
Contact name X.Z Shawn Xu
E-mail(s) shawnxu@umich.edu
Phone 734-615-9311
Organization name Life Sciences Institute, University of Michigan
Street address 210 Washtenaw Ave, Room 6115A
City Ann Arbor
State/province Michigan
ZIP/Postal code 48109
Country USA
 
Platforms (1)
GPL19291 Affymetrix C. elegans Gene 1.1 ST Array
Samples (24)
GSM1524926 WT Adult at 15, prep1
GSM1524927 WT Adult at 15, prep2
GSM1524928 WT Adult at 15, prep3
Relations
BioProject PRJNA263895

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62297_RAW.tar 112.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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