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Series GSE62146 Query DataSets for GSE62146
Status Public on Nov 07, 2014
Title Rapid neurogenesis through transcriptional activation in human stem cell (Agilent)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Advances in cellular reprogramming and stem cell differentiation now enable ex vivo studies of human neuronal differentiation. However, it remains challenging to elucidate the underlying regulatory programs because differentiation protocols are laborious and often result in low neuron yields. Here, we overexpressed two murine Neurogenin transcription factors in human induced pluripotent stem cells, and obtained neurons with bipolar morphology in four days at greater than 90% purity. The high purity enabled mRNA and microRNA expression profiling during neurogenesis, thus revealing the genetic programs involved in the transition from stem cell to neuron. These profiles were then analyzed to identify the regulatory networks underlying the differentiation of the neurons. The arrays presented here assessed the differences between single neurogenin and dual neurogenin expression.
Overall design Gene expression profiling (microarray) of iPS cells (PGP1) at X days post- doxycycline induction of juman NEUROG1 and/or NEUROG2, or murine Neurog1 and Neurog2.
Contributor(s) Buskamp V, Lewis N, Patrick G
Citation(s) 25403753
Submission date Oct 07, 2014
Last update date Nov 27, 2018
Contact name Nathan Lewis
Organization name University of California, San Diego
Department Pediatrics
Street address 9500 Gilman Dr. 0760
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (16)
GSM1520340 PGP1 with Human NEUROG1 replicate 1
GSM1520341 PGP1 with Human NEUROG1 replicate 2
GSM1520342 PGP1 with Human NEUROG1 replicate 3
BioProject PRJNA263275

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62146_RAW.tar 202.8 Mb (http)(custom) TAR (of TXT)
Processed data not provided for this record

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