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Series GSE62073 Query DataSets for GSE62073
Status Public on Nov 21, 2014
Title Convergent evolution of complex regulatory landscapes and pleiotropy at Hox loci (tiling)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary Hox genes are required during the morphogenesis of both vertebrate digits and external genitals.We investigated whether transcription in such distinct contexts involves a shared enhancer-containing landscape. We show that the same regulatory topology is used, yet with some tissue-specific enhancer-promoter interactions, suggesting the hijacking of a regulatory backbone from one context to the other. In addition, comparable organizations are observed at both HoxA and HoxD clusters, which separated through genome duplication in an ancestral invertebrate animal.We propose that this convergent regulatory evolution was triggered by the pre-existence of some chromatin architecture, thus facilitating the subsequent recruitment of the appropriate transcription factors. Such regulatory topologies may have both favored and constrained the evolution of pleiotropic developmental loci in vertebrates.
 
Overall design Chromatin ImmoPrecipitation on chip (Tiling array): Distribution of H3K27ac and H3K27me3 in WT genital tubercle (GT) at E13.5 and E15.5 Distribution of H3K27ac WT forelimb autopods are from Montavon et al., 2011.
 
Contributor(s) Lonfat N, Montavon T, Darbellay F, Gitto S, Duboule D
Citation(s) 25414315
Submission date Oct 06, 2014
Last update date Feb 25, 2015
Contact name Nicolas Lonfat
Organization name EPFL - Ecole Polytechnique Fédérale de Lausanne
Department School of Life Sciences
Lab Laboratory of developmental genomics
Street address EPFL-SV-ISREC-UPDUB- Station 19
City Lausanne
ZIP/Postal code 1015
Country Switzerland
 
Platforms (1)
GPL14183 Mus musculus HoxD cluster tiling PRNFGMm2b520462F
Samples (4)
GSM1519349 GT E15-5 H3K27Ac
GSM1519350 GT E15-5 H3K27Me3
GSM1519351 GT E13-5 H3K27Ac
This SubSeries is part of SuperSeries:
GSE62269 Convergent evolution of complex regulatory landscapes and pleiotropy at Hox loci
Relations
BioProject PRJNA263165

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62073_RAW.tar 7.0 Mb (http)(custom) TAR (of BAR, CEL)
Processed data provided as supplementary file

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