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Series GSE61995 Query DataSets for GSE61995
Status Public on Nov 02, 2014
Title m6A mRNA Methylation Facilitates Resolution of Naïve Pluripotency Towards Differentiation (m6a-seq)
Organism Mus musculus
Experiment type Other
Summary Naïve and primed pluripotent states retain distinct molecular properties, yet limited knowledge exists on how their state transitions are regulated. Here we identify Mettl3, an N6-Methyladenosine (m6A) transferase, as a regulator for terminating murine naïve pluripotency. Mettl3 knockout pre-implantation epiblasts and naïve embryonic stem cells (ESCs) are depleted for m6A in mRNAs and yet, are viable. However, they fail to adequately terminate their naïve state, and subsequently undergo aberrant and restricted lineage priming at the post-implantation stage, leading to early embryonic lethality. m6A predominantly and directly reduces mRNA stability, including that of key naïve pluripotency promoting transcripts. This study highlights a critical role for an mRNA epigenetic modification in vivo, and identifies regulatory modules that functionally influence naïve and primed pluripotency in an opposing manner.
 
Overall design m6A-seq was measured from total RNA in mouse embryonic stem cells (ESCs), embroid bodies (EBs) and embronic fibroblasts (MEF). 3 biological replicates are available from BVSC ESC line and EBs, and two biological replicates are available for MEFs. m6A-seq was also measured from total RNA in Mettl3 knockout ESCs. Each sample consist of IP to m6A and control input.
 
Contributor(s) Novershtern N, Moshitch-Moshkovitz S, Geula S, Kol N, Rechavi G, Hanna J
Citation(s) 25569111, 34893620
Submission date Oct 02, 2014
Last update date Jan 10, 2022
Contact name Noa Novershtern
E-mail(s) noa.novershtern@weizmann.ac.il
Organization name Weizmann Institute of Science
Department Molecular Genetics
Street address Weizmann Institute
City Rehovot
ZIP/Postal code 7610001
Country Israel
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (18)
GSM1518024 m6A-seq in ESCs, replicate A: IP
GSM1518025 m6A-seq in ESCs, replicate A: Input
GSM1518026 m6A-seq in ESCs, replicate B: IP
This SubSeries is part of SuperSeries:
GSE61998 m6A mRNA Methylation Facilitates Resolution of Naïve Pluripotency Towards Differentiation
Relations
BioProject PRJNA262906
SRA SRP048596

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Supplementary file Size Download File type/resource
GSE61995_RAW.tar 1.1 Mb (http)(custom) TAR (of BED)
GSE61995_final_peaks.xlsx 1.2 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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