GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE61938 Query DataSets for GSE61938
Status Public on Oct 02, 2014
Title Gene Expression in induced Treg cell from CD4+ Naive T cell isolated from miR-31fl/fl/CD4Cre vs Control mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary A microarray study performed in iTreg of miR-31fl/fl/CD4Cre and control mice to identify genes that are regulated by the miR-31. CD4+ naïve T cells from miR-31fl/fl mice and miR-31fl/flCD4Cre mice were used to induce iTreg in vitro. Four independent experiments were performed.
Overall design MiR-31fl/fl/CD4Cre mice (6 wk old) and age-matched control mice were sacrificed, and the spleen were removed and teased into cell-single suspensions and filtered through a 40 μm cell strainer. Naïve CD4+CD25-Foxp3gfp-CD62Lhi T cells were sorted by FACSAria III (BD bioscience).The medium used for T cell cultures was RPMI-1640 (Gibco) supplemented with 10% heat-inactivated FBS (Gibco), 2 mM L-glutamine (Gibco), 100 U/ml penicillin, 100 μg/ml streptomycin, and 5 mM β-mercaptoethanol (Gibco). The CD4+ Naïve T cells were stimulated with plate-bound anti-CD3 (5 μg/ml) plus soluble anti-CD28 (2 μg/ml) under iTreg cell differentiation conditions (mTGF-β1, 5 ng/ml and rmIL-2, 40 ng/ml; R&D Systems). After 4 days, harvested cell ,the RNA was extracted in Trizol and then purified using the miRNeasy Micro Kit (Qiagen). This submission shows the data obtained from 3 individual miR-31fl/fl/CD4Cre mice mice measured against 3 control mice.
Contributor(s) Zhang L, Wang H
Citation(s) 26165721
Submission date Oct 01, 2014
Last update date Apr 07, 2020
Contact name Lingyun Zhang
Organization name Shanghai Institute of Immunology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine
Department Shanghai Institute of Immunology, Institute of Medical Sciences
Lab Wang Honglin Lab
Street address 280 Chongqing South Road
City Shanghai
ZIP/Postal code 200025
Country China
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (6)
GSM1517490 CKO_iTreg_4 Days_rep1
GSM1517491 CKO_iTreg_4 Days_rep2
GSM1517492 CKO_iTreg_4 Days_rep3
BioProject PRJNA262717

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61938_RAW.tar 55.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap