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Series GSE61931 Query DataSets for GSE61931
Status Public on Oct 01, 2014
Title Adenylation of maternally inherited microRNAs by Wispy
Organisms Drosophila melanogaster; Danio rerio
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Early development depends heavily on accurate control of maternally inherited mRNAs, and yet it remains unknown how maternal microRNAs (miRNAs) are regulated during maternal to zygotic transition (MZT). We here find that maternal miRNAs are highly adenylated at their 3' ends in mature oocytes and early embryos. Pervasive adenylation is observed in oocytes of fly, sea urchin and mouse, indicating that maternal miRNA adenylation may be widely conserved in animals. We identify Wispy as the enzyme responsible for miRNA adenylation in flies. Wispy is known to be expressed specifically in oocytes and early embryos and function as a noncanonical poly(A) polymerase. Knockout of wispy abrogates miRNA adenylation and induces miRNA accumulation in fly eggs whereas overexpression of Wispy increases adenylation and reduces miRNA levels in S2 cells. Adenylation occurs on both the 5p and 3p miRNAs, indicating that Wispy acts on miRNAs after Dicer processing. We further find that Wispy interacts with Ago1 through protein-protein interaction, which may allow the effective and selective adenylation of miRNAs. Thus, adenylation may contribute to the clearance of maternally deposited miRNAs during MZT. Our work provides the first mechanistic insights into the regulation of maternal miRNAs and illustrates the importance of RNA tailing in development.
Overall design MiRNA expression and modification profile during early embryo development of fruit fly and zebra fish using high throughput sequencing
Contributor(s) Lee M, Choi Y, Kim K, Jin H, Lim J, Jeong M, Giraldez AJ, Kim VN
Citation(s) 25454948
Submission date Sep 30, 2014
Last update date May 15, 2019
Contact name Yeon Choi
Phone +82-2-887-1343
Organization name Seoul National University
Department School of Biological Sciences
Lab Narry Kim Lab
Street address Building 504 Room 501, School of Biological Sciences, Seoul National University, 1 Gwanangno, Gwanak-gu
City Seoul
ZIP/Postal code 151-742
Country South Korea
Platforms (3)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
GPL14875 Illumina HiSeq 2000 (Danio rerio)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
Samples (21)
GSM1517403 dme_w1118_0_2h
GSM1517404 dme_wispy_0_2h
GSM1517405 dme_Embryo_15m
BioProject PRJNA262696
SRA SRP048545

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Supplementary file Size Download File type/resource
GSE61931_RAW.tar 40.0 Kb (http)(custom) TAR (of CSV)
GSE61931_dme_miRNA-TMM.csv.gz 22.8 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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