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Series GSE61878 Query DataSets for GSE61878
Status Public on Dec 17, 2015
Title Genome wide binding of glucocorticoid receptor in dexamethasone treated human monocyte derived macrophages
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Macrophages are amongst the major targets of glucocorticoids (GC) as therapeutic anti-inflammatory agents. Here we show that GC treatment of mouse and human macrophages initiates a cascade of induced gene expression including many anti-inflammatory genes. Inducible binding of the glucocorticoid receptor (GR) was detected at candidate enhancers in the vicinity of induced genes in both species and this was strongly associated with canonical GR binding motifs. However, the sets of inducible genes, the candidate enhancers, and the GR motifs within them, were highly-divergent between the two species.. The data cast further doubt upon the predictive value of mouse models of inflammatory disease.
Overall design human monocyte derived macrophages were treated with dexamethasone for 2h, fixed and ChIP performed. Material from 4 volunteers was pooled for the IP.
Contributor(s) Jubb AW, Bickmore WA, Hume DA, Young RS
Citation(s) 26663721, 29241532
Submission date Sep 29, 2014
Last update date Jul 15, 2019
Contact name Alasdair W Jubb
Organization name University of Edinburgh
Department Roslin Institute
Lab Hume lab / Bickmore lab (MRC HGU)
Street address The Roslin Institute
City Easter Bush
State/province Midlothian
ZIP/Postal code EH25 9RG
Country United Kingdom
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (2)
GSM1516132 hMDM_GR_IP
GSM1516133 hMDM_GR_input
This SubSeries is part of SuperSeries:
GSE61881 Divergent transcriptional activation by glucocorticoids in mouse and human macrophages is the result of gain and loss of enhancers
BioProject PRJNA262586
SRA SRP048473

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Supplementary file Size Download File type/resource
GSE61878_hMDM_GR_ChIP_dex100nM_2h_pooled_peaks.txt.gz 17.6 Kb (ftp)(http) TXT
GSE61878_hMDM_GR_ChIP_dex100nM_2h_pooled_ucsc.bedGraph.gz 27.4 Mb (ftp)(http) BEDGRAPH
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