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Series GSE61825 Query DataSets for GSE61825
Status Public on Jun 08, 2016
Title Emergence of neural crest identity is a key early step in melanoma formation [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The “cancerized field” concept posits that cells of a given tissue share a potentially oncogenic mutation or insult and are thus cancer-prone, yet only discreet clones within the field initiate tumor formation. In melanoma, tumors frequently (~50%) carry the oncogenic BRAFV600E mutation that is also nearly always present in benign nevi that rarely become melanoma. The zebrafish crestin gene is expressed embryonically in neural crest progenitors and is then specifically re-expressed in melanoma tumors. Here, we show by live imaging transgenic zebrafish crestin reporters that single melanocytes in a cancerized field with oncogenic BRAFV600E and p53 tumor suppressor loss undergo a change that recapitulates the neural crest progenitor (NCP) state, and patches of these cells initiate early melanoma. The crestin element is regulated by the NCP transcription factor sox10. Forced sox10 overexpression in melanocytes accelerated melanoma formation, consistent with reprogramming to the NCP state and activation of super-enhancers that lead to melanoma. Our work highlights the importance of the reemergence of the NCP state as a barrier to melanoma initiation.
Overall design ChIP-seq analysis of histone 3 lysine 27 acetylation in human A375 melanoma cells treated with DMSO
Contributor(s) Kaufman CK, Yang S, Zhou Y, Zon LI
Citation(s) 26823433
Submission date Sep 28, 2014
Last update date May 15, 2019
Contact name Leonard Zon
Organization name Boston Children's Hospital
Department Oncology/Hematology
Street address 1 Blackfan Circle
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (2)
GSM1515413 Input on DMSO-treated cells
GSM1515414 H3k27ac ChIP on DMSO-treated cells
This SubSeries is part of SuperSeries:
GSE75356 A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation
BioProject PRJNA262500
SRA SRP048200

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Supplementary file Size Download File type/resource
GSE61825_RAW.tar 52.7 Mb (http)(custom) TAR (of BW)
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Processed data provided as supplementary file
Raw data are available in SRA

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