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Series GSE61808 Query DataSets for GSE61808
Status Public on Sep 25, 2015
Title Nanos2-regulated mRNAs in the cultured spermatogonial stem cells (SSCs)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Spermatogonial stem cells are foundation of spermatogenesis. In our previous study, we observed a rapid degeneration of spermatogenesis by conditional knockout (cKO) of Nanos2 in adult spermatogonia, but the underlying mechanisms are largely unknown. While, conditional overexpression (cOE) of Nanos2 also inhibited germ cell development and stayed in undifferentiation status. Here we used cultured germline stem cells (GSCs) to study the mechanisms of Nanos2 in spermatogonia. We compared Nanos2 expression in cultured GSCs with in vivo isolated Nanos2 positive cells, and found the level of Nanos2 is significantly lower in GSCs as compared to Nanos2 positive cells. Thus we generated Nano2 cOE GSCs lines. By conditional inducing expression of Nanos2 we compared gene expression profile to control GSCs. These data provide the first evidence of Nanos2 regulating genes in spermatogonial stem cells.
Overall design Ectopic induction of Nanos2 in cultured GSCs. Duplicated control (Roa-CreERT2;CAG-CAT-3FlagNanos2-pA, DMSO) and experiment (Roa-CreERT2;CAG-CAT-3FlagNanos2-pA, Tamoxifen)
Contributor(s) Zhou Z, Yumiko S
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Submission date Sep 26, 2014
Last update date Jan 12, 2017
Contact name ZHI ZHOU
Phone 055-981-6832
Organization name National Institute of Genetics, Japan
Department Division of Mammalian Development
Lab Mammalian Development laboratory
Street address Yata 1111
City Mishima
ZIP/Postal code 4118540
Country Japan
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (4)
GSM1514380 Nanos2_conditional_overexpression_Germline_stem_cells_treated_with_DMSO_1
GSM1514381 Nanos2_conditional_overexpression_Germline_stem_cells_treated_with_DMSO_2
GSM1514382 Nanos2_conditional_overexpression_Germline_stem_cells_treated_with_4-Tamoxifen_1
BioProject PRJNA262174

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61808_RAW.tar 36.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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