GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE61744 Query DataSets for GSE61744
Status Public on Jan 01, 2016
Title Genome-wide profiling of PARP1 reveals an interplay with gene regulatory regions and DNA methylation
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary To address the global impact of PARP-1 on DNA methylation, we treated cells with PJ34 (PARylation inhibitor) and isolated genomic DNA from vehicle and PJ34 treated cells. This DNA was bisulfite treated and hybridized to the Illumina infinium Methylation 450 Beadchip. We next used these RNA-seq data sets (control, PARP-1 KD and PARylation inhibited) to assess whether PARP plays a role in DNA methylation by assessing differential methylation patterns.
PARP1 mediates methylation patterns.
Overall design DNA from vehicle and PJ34 (PARylation inhibited) cells. 750ng of geneomic DNA was bisulfite converted and used for the Illumia infinium HD methylation assay.
Contributor(s) Nalabothula N, Taha A, Shao X, Fondufe-Mittendorf Y
Citation(s) 26305327
Submission date Sep 25, 2014
Last update date Mar 22, 2019
Contact name Yvonne Nsokika Fondufe-Mittendorf
Phone 8593230091
Organization name University of Kentucky
Department Biochemistry
Lab Mittendorf
Street address 741 South Limestone dr.
City Lexington
State/province KY
ZIP/Postal code 40536-0509
Country USA
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (2)
GSM1513282 DNA-methylation in PJ-34 treated cells
GSM1513283 DNA methylation in NT cells
BioProject PRJNA262012

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61744_RAW.tar 183.1 Mb (http)(custom) TAR
GSE61744_signals.txt.gz 6.7 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap