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| Status |
Public on Sep 23, 2014 |
| Title |
Twi1p-loaded scnRNAs in GIW1 KO Tetrahymena cells |
| Organism |
Tetrahymena thermophila |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To understand the importance of Giw1p in the loading of small RNAs to the Argonaute protein Twi1p in Tetrahymena, we compared Twi1p-bound small RNAs from wild-type cells and GIW1 KO cells. We found that the Twi1p-bound small RNA populations in wild-type cells and GIW1 KO cells are indistinguishable in their size distributions and base profiles.
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| Overall design |
We immunopurified Twi1p-containing complex from wild-type and GIW1 KO cells and co-purified small RNAs were compared.
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| Contributor(s) |
Noto T, Mochizuki K |
| Citation(s) |
25588944 |
| |
| Submission date |
Sep 22, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Kazufumi Mochizuki |
| E-mail(s) |
kazufumi.mochizuki@igh.cnrs.fr
|
| Organization name |
Institute of Human Genetics, CNRS
|
| Street address |
141 rue de la Cardonille
|
| City |
Montpellier |
| ZIP/Postal code |
34090 |
| Country |
France |
| |
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| Platforms (1) |
| GPL15706 |
Illumina HiSeq 2000 (Tetrahymena thermophila) |
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| Samples (2) |
| GSM1509373 |
Twi1p-bound 16-32-nt small RNAs from wild-type cells at 3 hpm |
| GSM1509374 |
Twi1p-bound 16-32-nt small RNAs fromGIW1 KO cells at 3 hpm |
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| Relations |
| BioProject |
PRJNA261680 |
| SRA |
SRP047373 |
