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| Status |
Public on Sep 17, 2014 |
| Title |
Gene expression profiles in rat lung following intratracheal instillation with impurity-free single-wall carbon nanotubes |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
To further development of our gene expression approach to assess the effects of manufactured nanomaterials at the transcriptional level, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish characterization of physicochemical properties of impurity-free single-wall carbon nanotubes (SWCNTs). We have prepared two types of dispersed the SWCNTs, namely relatively small bundles and a short linear shape (CNT-1) and large bundles and a long linear shape (CNT-2), and attempted to characterize time-dependent changes in the gene expression of lung tissues until 90 days after intratracheal instillation with SWCNTs suspensions at 0.4 mg injected dose per rat.
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| Overall design |
Groups of nine-week-old male Wistar rats (n= 4 per group/ time point) were intratracheally instilled with single-wall carbon nanotubes (SWCNTs) suspended in 0.4 ml of 1.0mg/mL bovine serum albumin (BSA) as a single injection 0.4 mg SWCNTs/ rat. Control groups received 1.0mg/mL BSA (vehicle control). After intratracheal instillation treatment, rats were housed within polycarbonate cages at a controlled temperature of 22 °C with a chow diet ad libitum, and dissected at 1day, 3 days, 7 days, 30 days, and 90 days post-instillation. Right lungs of anesthetized rats were perfused with physiological saline, excised, and used for DNA microarray analysis.
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| Contributor(s) |
Fujita K, Fukuda M |
| Citation(s) |
25865113 |
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| Submission date |
Sep 17, 2014 |
| Last update date |
Apr 16, 2015 |
| Contact name |
Katsuhide Fujita |
| E-mail(s) |
ka-fujita@aist.go.jp
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| Organization name |
National Institute of Advanced Industrial Science and Technology
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| Department |
Research Institute of Science for Safety and Sustainability
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| Street address |
Onogawa 16-1
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| City |
Tsukuba |
| ZIP/Postal code |
3058569 |
| Country |
Japan |
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| Platforms (1) |
| GPL7294 |
Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Probe Name version) |
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| Samples (15)
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| GSM1506002 |
Lung_Control, 3days |
| GSM1506003 |
Lung_CNT-1, 3days |
| GSM1506004 |
Lung_CNT-2, 3days |
| GSM1506005 |
Lung_Control, 7days |
| GSM1506006 |
Lung_CNT-1, 7days |
| GSM1506007 |
Lung_CNT-2, 7days |
| GSM1506008 |
Lung_Control, 30days |
| GSM1506009 |
Lung_CNT-1, 30days |
| GSM1506010 |
Lung_CNT-2, 30days |
| GSM1506011 |
Lung_Control, 90days |
| GSM1506012 |
Lung_CNT-1, 90days |
| GSM1506013 |
Lung_CNT-2, 90days |
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| Relations |
| BioProject |
PRJNA261287 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE61483_RAW.tar |
566.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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