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Series GSE61330 Query DataSets for GSE61330
Status Public on Oct 21, 2014
Title Role of Tet3 and DNA replication in zygotic demethylation of both paternal and maternal genomes [RRBS]
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary With the exception of imprinted genes and certain repeats, DNA methylation is globally erased during pre-implantation development. Recent studies have suggested that Tet3-mediated oxidation of 5-methylcytosine (5mC) and DNA replication-dependent dilution both contribute to global paternal DNA demethylation, but demethylation of the maternal genome occurs via replication. Here we present genome-scale DNA methylation maps for both the paternal and maternal genomes of Tet3-depleted and/or DNA replication-inhibited zygotes. In both genomes, we found that inhibition of DNA replication blocks DNA demethylation independently from Tet3 function, and that Tet3 facilitates DNA demethylation by coupling with DNA replication. For both, our data indicate that replication-dependent dilution is the major contributor to demethylation, but Tet3 plays an important role, particularly at certain loci. Our study therefore both defines the respective functions of Tet3 and DNA replication in paternal DNA demethylation and reveals an unexpected contribution of Tet3 to demethylation of the maternal genome.
Overall design In this data set, we include RRBS data of manually isolated paternal and maternal pronuclei from both WT and Tet3 CKO zygotes with or without aphidicolin treatment
Contributor(s) Shen L, Inoue A, He J, Liu Y, Lu F, Zhang Y
Citation(s) 25280220
Submission date Sep 11, 2014
Last update date May 15, 2019
Contact name Li Shen
Organization name HHMI/Boston Children's Hospital/Harvard Medical School
Street address 200 Longwood Ave
City Boston
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (20)
GSM1502478 CKO-Mat-Aphi-rep1
GSM1502479 CKO-Mat-Aphi-rep2
GSM1502480 CKO-Mat-rep1
This SubSeries is part of SuperSeries:
GSE61331 Role of Tet3 and DNA replication in zygotic demethylation of both paternal and maternal genomes
BioProject PRJNA260794
SRA SRP047032

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Supplementary file Size Download File type/resource
GSE61330_RAW.tar 176.2 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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