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Series GSE61247 Query DataSets for GSE61247
Status Public on Apr 29, 2015
Title ChIP-seq profiling of loci bound by HIF1a in wild-type E12.5 hearts
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Purpose: to identify genes directly bound by HIF1a in cardiomycoytes
Methods: crosslinked chromatin isolated from E12.5 mouse hearts was precipitated using an anti-HIF1a antibody and processed for sequencing. A list of genes containing a HIF1a peak (Minimum score 3.5) was compared with RNA-seq results to identify genes that are bound by HIF1a and expressed in E12.5 hearts
Results: this analysis identified 1016 genes expressed and bound by HIF1a in embryonic hearts
Overall design Two experimental replicates and one input
Contributor(s) GuimarĂ£es-Camboa N, Carlos Zambon A
Citation(s) 26028220
Submission date Sep 09, 2014
Last update date May 15, 2019
Contact name Alexander C Zambon
Organization name Keck Graduate Institute
Department Biopharmaceutical Sciences
Lab Zambon
Street address 517 Watson Drive
City Claremont
State/province CA
ZIP/Postal code 91711
Country USA
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (3)
GSM1500749 Input
GSM1500750 Hif1a pulldown replicate 1
GSM1500751 Hif1a pulldown replicate 2
BioProject PRJNA260618
SRA SRP046747

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61247_cIP1cIP2.mergepeaks.bed.gz 245.0 Kb (ftp)(http) BED
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Raw data are available in SRA
Processed data are available on Series record

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