GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE61181 Query DataSets for GSE61181
Status Public on Sep 06, 2014
Title Islet-specific microRNA changes driven by nutrient shift trigger postnatal beta-cell maturation - dispersed islet cell mRNAs after miR-17-5p transfection
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary We found that in rodents, postnatal beta-cell maturation is associated with changes in the expression of several islet microRNAs and discovered that these modifications are driven by changes in the nutrient supply. Mimicking the microRNA changes observed during β-cell maturation in newborn rat islet cells was sufficient to promote glucose-induced insulin release and to achieve a mature β-cell secretory phenotype. Moreover, the modifications in the level of some of these microRNAs reduced the proliferation of newborn β-cells, suggesting that they contribute to the limited proliferative capacity of adult β-cells. These findings demonstrated that miRNAs contribute to postnatal beta-cell maturation and development. Their role is likely to promote beta-cell adaptation to fule supply and to maintain glucose homeostasis by regulating insulin release and proliferation.
Overall design Islets from 10-day-old rats (P10) were taken, dispersed and transfected with control miRNA mimic or miR-17-5p. Total RNA was extracted and mRNA profiling via Illumina single-end sequencing of mRNA-seq libraries was performed. In parallel, Ago2 immunoprecipitation with RNA recovery and mRNA-seq was performed (RISC-seq).
Contributor(s) Matkovich SJ, Jacovetti C, Regazzi R
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Sep 05, 2014
Last update date May 15, 2019
Contact name Scot J Matkovich
Organization name Eli Lilly and Company
Street address Lilly Corporate Center
City Indianapolis
State/province IN
ZIP/Postal code 46285
Country USA
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (15)
GSM1498968 control-transfected_P10-1_mRNA
GSM1498969 control-transfected_P10-2_mRNA
GSM1498970 control-transfected_P10-3_mRNA
This SubSeries is part of SuperSeries:
GSE61182 MicroRNA and mRNA expression analyses of postnatal and adult rat pancreatic islets
BioProject PRJNA260414
SRA SRP046247

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61181_RAW.tar 1000.0 Kb (http)(custom) TAR (of TXT)
GSE61181_islet_P10tf_RISC_summary.txt.gz 171.6 Kb (ftp)(http) TXT
GSE61181_islet_P10tf_mRNA_summary.txt.gz 207.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap