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Series GSE61136 Query DataSets for GSE61136
Status Public on Oct 07, 2015
Title Genome wide map of heterochromatin state in fission yeast Schizosaccharomcyces pombe (S. pombe)
Organism Schizosaccharomyces pombe
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Proteins of the conserved HP1 family are elementary components of heterochromatin and are generally assumed to play a central role in creating a rigid heterochromatic network that is densely packed and inaccessible to the transcription machinery. In this study we demonstrate that the fission yeast HP1 protein Swi6 exists as a single highly dynamic population and rapidly exchanges in cis and in trans between different heterochromatic regions. Binding to methylated H3K9 or to heterochromatic RNA decelerates Swi6 mobility. We further show that Swi6 is largely dispensable to maintain heterochromatin domains. In contrast, our results disclose an unexpected role of Swi6 in demarcating constitutive heterochromatin from neighboring euchromatin. Our results are consistent with a stochastic model of heterochromatin and imply that heterochromatin is permissive for transcription throughout the cell cycle. Rather than promoting maintenance and spreading of heterochromatin, Swi6 appears to limit these processes to ensure that heterochromatin is appropriately confined.
 
Overall design Examination of a single histone modification in a Swi6 deletion fission yeast strain
 
Contributor(s) Kulasegaran-Shylini R, Gaidatzis D, Dessus-Babus S, Buehler M
Citation(s) 26438724
Submission date Sep 04, 2014
Last update date May 15, 2019
Contact name Tim Roloff
E-mail(s) tim.roloff@fmi.ch
Organization name FMI
Department Functional Genomics
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platforms (1)
GPL13988 Illumina HiSeq 2000 (Schizosaccharomyces pombe)
Samples (2)
GSM1498232 Swi6D_H3K9me2_ChIPSEQ_rep1
GSM1498233 Swi6D_H3K9me2_ChIPSEQ_rep2
This SubSeries is part of SuperSeries:
GSE70946 H3K9 methylation extends across natural boundaries of heterochromatin in the absence of an HP1 protein
Relations
BioProject PRJNA260380
SRA SRP046225

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61136_Swi6deletion.wig.gz 1.1 Mb (ftp)(http) WIG
GSE61136_pombe_09052011.gff.gz 1.9 Mb (ftp)(http) GFF
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Raw data are available in SRA
Processed data are available on Series record

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