|
| Status |
Public on Apr 07, 2015 |
| Title |
Development of gene expression signatures for miR-340 overexpressing human glioma-initiating cells (GICs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify functional targets of miR-340, we compared the gene expression profiles of miR-340 overexpressing human GICs with the profiles of their parental cells.
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| |
|
| Overall design |
The precursor form of miR-340 or control miRNA was overexpressed in glioma cells using miRNA lentiviral particles. The cells were incubated with recombinant virus at 37°C for 12 hr and cultured in the presence of puromycin for 3 days.
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| |
|
| Contributor(s) |
Yamashita D, Kondo T |
| Citation(s) |
25627976 |
| Submission date |
Sep 03, 2014 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Daisuke Yamashita |
| E-mail(s) |
yamadai551208@gmail.com
|
| Organization name |
Ehime University Graduate School of Medicine
|
| Department |
Neurosurgery
|
| Street address |
Shitsukawa
|
| City |
Toon |
| ZIP/Postal code |
791-0295 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
|
| Samples (11)
|
| GSM1496287 |
DA: glioma-initiating cells (GICs) |
| GSM1496288 |
AO: glioma-initiating cells (GICs) |
| GSM1496289 |
U215: glioma cell lines |
| GSM1496290 |
U87: glioma cell lines |
| GSM1496291 |
GBM-E2-miR340: miR340 overexpressed glioma-initiating cells (GICs) |
| GSM1496292 |
GBM-E3-miR340: miR340 overexpressed glioma-initiating cells (GICs) |
| GSM1496293 |
GBM-E5-miR340: miR340 overexpressed glioma-initiating cells (GICs) |
| GSM1496294 |
U251-miR340: miR340 overexpressed glioma cell lines |
|
| Relations |
| BioProject |
PRJNA260196 |
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