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Series GSE61017 Query DataSets for GSE61017
Status Public on Sep 03, 2014
Title Gene expression profile of HoxA9 over expressing and empty vector (EV) control Hemato-Endothelial Progenitors (HEPs) subpopulations
Organism Homo sapiens
Experiment type Expression profiling by array
Summary During hematopoietic differentiation of hESCs, HOXA9 expression parallels hematopoietic development but is restricted to the hemogenic precursors (HEP, CD31+CD34+CD45-), and diminishes as HEPs differentiate into blood cells (CD45+). Enforced expression of Hoxa9 in hESCs robustly promoted differentiation into primitive (CD34+CD45+) and total (CD45+) blood cells with higher clonogenic (CFU) potential. To identify patterns of gene expression that could explain at the molecular level the developmental impact of HOXA9 in hematopoietic commitment of hESCs, we performed gene expression profile in FACS-purified EV- and HOXA9-HEPs.
Overall design 10^5 HoxA9 over expressing and EV control HEPs purified by FACS from H9 and AND1 hEBs at day 15 of hematopoietic differentiation were used for gene expression analysis using Whole Human Genome Oligo Microarray chips (Agilent Technologies).
Contributor(s) Ramos-Mejia V, Menendez P
Citation(s) 25185710
Submission date Sep 02, 2014
Last update date Nov 27, 2018
Contact name Pedro Jose Real
Phone +34 958 71 55 00
Organization name GENyO
Department Molecular Oncology
Lab Gene Regulation, Stem Cells and Development
Street address Avda. Ilustracion 114
City Granada
State/province Granada
ZIP/Postal code 18016
Country Spain
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (3)
GSM1495574 H9 GFP EBD15 PFVs
GSM1495575 H9 HOXA9 EBD15 PFVs Rep 1
GSM1495576 H9 HOXA9 EBD15 PFVs Rep 2
BioProject PRJNA260082

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Supplementary file Size Download File type/resource
GSE61017_RAW.tar 41.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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