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Series GSE60872 Query DataSets for GSE60872
Status Public on Apr 28, 2016
Title The functional impact of RNA Polymerase II pausing across multiple mamamlian cell types
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We analyzed the breadth and functional relationships of RNA Polymerase II pausing across many human and mouse cell types to understand what roles RNA Polymerase II pausing plays in gene regulation. We identified a novel association of H2A.Z at the TSS of increasingly paused genes. We knocked down H2A.Z to test whether H2A.Z positively or negatively affects RNA Polymerase II pausing to find that pausing globally increased upon knockdown.
Overall design We tested the effect of H2A.Z knockdown on RNA Polymerase II pausing by setting up a case vs. control experiment in which MCF7 breast cancer cells were treated with a siRNA against H2A.Z. We tested for changes in RNA Polymerase II pausing as a result.
Contributor(s) Pu WT, Zhang B
Citation(s) 27259512
Submission date Aug 28, 2014
Last update date May 15, 2019
Contact name Peter J Park
Phone 617-432-7373
Organization name Harvard Medical School
Department Center for Biomedical Informatics
Street address 10 Shattuck St
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (3)
GSM1492701 Pol II Control
GSM1492702 Pol II H2AZ knockdown
GSM1492703 Input
BioProject PRJNA259708
SRA SRP045858

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Supplementary file Size Download File type/resource
GSE60872_RAW.tar 950.0 Kb (http)(custom) TAR (of CSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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