GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE60808 Query DataSets for GSE60808
Status Public on Apr 02, 2015
Title Jarid2 regulates hematopoietic stem cell function by acting with Polycomb Repressive Complex 2
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Polycomb Repressive Complex 2 (PRC2) has been shown to play a key role in hematopoietic stem and progenitor cell (HSPC) function. Analyses of mouse mutants harboring deletions of core components have implicated PRC2 in fine-tuning multiple pathways that instruct HSPC behavior, yet how PRC2 is targeted to specific genomic loci within HSPCs remains unknown. Here we use shRNA-mediated knockdown to survey the function of known PRC2 accessory factors in HSPCs by testing the competitive reconstitution capacity of transduced murine fetal liver cells. We find that similar to the phenotype observed upon depletion of core subunit Suz12, depleting Jarid2 enhances the competitive transplantation capacity of both fetal and adult, mouse and human HSPCs. Gene expression profiling revealed common Suz12 and Jarid2 target genes that are enriched for the H3K27me3 mark established by PRC2. These data implicate Jarid2 as an important component of PRC2 that has a central role in coordinating HSPC function.
Overall design RNA-seq of jarid knockdown, suz knockdown and control from HSPC in 16 week old mice.
Contributor(s) Kinkel SA, Blewitt ME
Citation(s) 25645357
Submission date Aug 27, 2014
Last update date May 15, 2019
Contact name Marnie Blewitt
Organization name WEHI
Department Molecular Medicine Division
Lab Blewitt Laboratory
Street address 1G Royal Parade
City Parkville
State/province VIC
ZIP/Postal code 3052
Country Australia
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (16)
GSM1489178 nons R1
GSM1489179 nons R2
GSM1489180 nons R3
BioProject PRJNA259600
SRA SRP045805

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60808_DifferentialExpression.csv.gz 1.1 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap