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Series GSE6080 Query DataSets for GSE6080
Status Public on Jul 01, 2007
Title JAM-A Knockdown in HepG2 Cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Hepatocytes are polarized epithelial cells whose function depends upon their ability to distinguish between the apical and basolateral surfaces that are located at intercellular tight junctions. It has been proposed that the signaling cascades that originate at these junctions influence cellular activity by controlling gene expression in the cell nucleus. To assess the validity of this proposal with regard to hepatocytes, we depleted expression of the tight junction protein junctional adhesion molecule-A (JAM-A) in the HepG2 human hepatocellular carcinoma cell line. Reduction of JAM-A resulted in a striking change in cell morphology, with cells forming single-layered sheets instead of the normal multi-layered clusters. In the absence of JAM-A, other tight junction proteins were mislocalized, and canaliculi, which form the apical face of the hepatocyte, were consequently absent. While most changes in gene expression were modest, there was a strong transcriptional induction of the adherens junction protein E-cadherin in cells with reduced levels of JAM-A. This increase in E-cadherin was partially responsible for the observed alterations in cell morphology and mislocalization of tight junction proteins. We therefore propose that we have uncovered a novel mechanism for crosstalk between specific components of tight and adherens junctions that can be utilized to regulate adhesion between hepatic cells and to maintain hepatocyte cell polarity.
Keywords: HepG2 cells
Overall design HepG2 cells were transduced with lentiviruses and stably selected using puromycin. Comparison of four controls and five JAM-A knockdown samples.
Contributor(s) Konopka G, Duncan S
Citation(s) 17623668
Submission date Oct 18, 2006
Last update date Dec 06, 2018
Contact name Stephen A Duncan
Phone 843-792-9104
Organization name Medical University South Carolina
Department Regenerative Medicine and Cell Biology
Lab Duncan Lab
Street address 173 Ashley Avenue, BSB 6th Floor, Room 657A
City Charleston
State/province SC
ZIP/Postal code 29425
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (9)
GSM140868 HepG2 FA
GSM140869 HepG2 FA2
GSM140870 HepG2 Vector
BioProject PRJNA97565

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Supplementary file Size Download File type/resource
GSE6080_RAW.tar 20.1 Mb (http)(custom) TAR (of CEL)

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