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Series GSE60777 Query DataSets for GSE60777
Status Public on Sep 01, 2016
Title Active Demethylation of Both Maternal and Paternal Genomes in Mouse Zygotes
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary The epigenomes of mammalian sperm and oocytes, characterized by gamete-specific 5-methylcytosine (5mC) patterns, are reprogrammed in early embryogenesis to establish full developmental potential. It is broadly accepted that the paternal genome is actively demethylated in the zygote while the maternal genome undergoes passive demethylation thanks to DNA replication over the subsequent cleavage divisions. Here we reveal that both maternal and paternal genomes undergo widespread active and passive demethylation in the pronuclear zygote before the first mitotic division. Whereas the passive demethylation requires DNA replication, the active demethylation relies on enzymatic oxidation of 5mC, as deletion of the DNA dioxygenase, Tet3, but not the inhibition of replication, blocks the active demethylation. At actively demethylated loci, 5mCs appear to be processed to unmodified cytosines in a manner independent of the DNA glycosylase TDG. These observations suggest the occurrence of genuine active demethylation in both parental genomes following fertilization.
Overall design An extra supportive Tet3 knock-out female pronuclear sample related to experiment Series GSE56650.
Contributor(s) Guo F, Li X, Liang D, Li T, Zhu P, Guo H, Wu X, Wen L, Gu TP, Hu B, Walsh CP, Li JS, Tang F, Xu GL
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Submission date Aug 26, 2014
Last update date May 15, 2019
Contact name Xianlong Li
Organization name Peking University
Department Biodynamic Optical Imaging Center, College of Life Sciences
Street address 5, Yiheyuan Rd., Haidian District
City Beijing
ZIP/Postal code 100871
Country China
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (1)
GSM1366374 FemalePN_T3KO_2
BioProject PRJNA259532
SRA SRP045826

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Supplementary file Size Download File type/resource
GSE60777_RAW.tar 1.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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