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Series GSE60715 Query DataSets for GSE60715
Status Public on Aug 27, 2014
Title Cells and exosomes after cell-free culture
Platform organism synthetic construct
Sample organisms Homo sapiens; Mus musculus
Experiment type Non-coding RNA profiling by array
Summary The goal of this study is to report that breast cancer associated exosomes contain microRNAs (miRNAs) associated with the RISC Loading Complex (RLC) and display cell-independent capacity to process precursor microRNAs (pre-miRNAs) into mature miRNAs. Pre-miRNAs, along with Dicer, AGO2, and TRBP, are present in exosomes of cancer cells. CD43 mediates the accumulation of Dicer specifically in cancer exosomes. Cancer exosomes mediate an efficient and rapid silencing of mRNAs to reprogram the target cell transcriptome. Exosomes derived from cells and sera of patients with breast cancer instigate non-tumorigenic epithelial cells to form tumors in a Dicer-dependent manner. These findings offer opportunities for the development of exosomes based biomarkers and therapies.
Overall design Exosomes from cancer cells and non-tumorigenic cells were isolated using established ultracentrifugation methods. The global miRNA content of cancer exosomes [MCF7 exos, MDA-MB-231 exos and 4T1 exos] and normosomes [NmuMG exos and MCF10A exos] was investigated before and after exosomes were cultured in cell-free media to study the microRNA biogenesis in exosomes. Samples 1 and 2 are samples with different times of culture [72hr and 24hrs, respectively].
Contributor(s) Melo SA, Kalluri R
Citation(s) 25446899
Submission date Aug 25, 2014
Last update date Jan 08, 2018
Contact name RAGHU KALLURI
Organization name MDACC
Street address 1881 East Rd.
City Houston
ZIP/Postal code 77054-1901 TX
Country USA
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (12)
GSM1486483 MCF7 cells_1
GSM1486484 MCF7 cells_2
GSM1486485 MCF7 exos_1
This SubSeries is part of SuperSeries:
GSE60716 Cell-Independent MicroRNA Biogenesis
BioProject PRJNA259429

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60715_RAW.tar 10.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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