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Series GSE60674 Query DataSets for GSE60674
Status Public on Jan 04, 2017
Title Upregulation of Interferon-inducible and damage response pathways in chronic graft-versus-host disease
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To identify systemic cytokine patterns in Chronic Graft-versus-Host-Disease (CGVHD), we profiled the gene expression of circulating monocytes. Pathway analysis identified two gene sets that were significantly upregulated across a broad range of patients with inflammatory and sclerotic presentations: (1) genes induced by Type I and Type II IFN, and (2) receptor genes for innate immune responses to cellular damage. Multiple IFN-inducible genes involved in signal transduction, anti-viral function, lymphocyte homeostasis, trafficking, and antigen presentation were increased. Furthermore, upregulation of TLR/NLR/CLR receptor genes for nucleic acids, ribonucleoproteins and annexin implicated response to damaged cells as a source of activation of inflammasomes and induction of Type I IFN.
Serial time courses substantiated a pattern of upregulation of multiple IFN-inducible genes at CGVHD onset, and of decline upon therapy and resolution. Corticosteroid effects could be discriminated from CGVHD gene profiles by assessment of glucocorticoid-inducible genes. Elevated expression of IFN-inducible proteins in plasma and tissue substantiated a role for IFN in lymphocyte trafficking and, through upregulation of BAFF, an involvement of IFN in B cell activation in CGVHD. These results support a unifying hypothesis of induction of IFN by innate response to cellular damage as a mechanism for initiation and persistence of CGVHD.
Overall design To identify systemic cytokine patterns in Chronic Graft-versus-Host-Disease (CGVHD), we profiled the gene expression of circulating monocytes
Contributor(s) Hakim FT, Memon S, Jin P, Imanguli M, Wang H, Rehman N, Yan X, Rose J, Mays JW, Dhamala S, Stroncek D, Gress RE
Citation(s) 27694491
Submission date Aug 22, 2014
Last update date Jan 25, 2019
Contact name PING JIN
Organization name CC/DTM/NIH
Department DTM
Street address 9000 ROCKVILLE PIKE
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL10739 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [probe set (exon) version]
Samples (36)
GSM1484401 CGVHD_1
GSM1484402 CGVHD_2
GSM1484403 ND_1
BioProject PRJNA259225

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Supplementary file Size Download File type/resource
GSE60674_RAW.tar 150.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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