GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE60655 Query DataSets for GSE60655
Status Public on Nov 04, 2014
Title DNA methylation analysis of human skeletal muscle after endurance training
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary Regular endurance exercise training induces beneficial functional and health effects in human skeletal muscle. The putative contribution to the training response of the epigenome as a mediator between genes and environment has not been clarified. Here we investigated the contribution of DNA methylation and associated transcriptomic changes in a well-controlled human intervention study. Training effects were mirrored by significant alterations in DNA methylation and gene expression in regions with a homogeneous muscle energetics and remodeling ontology. Differential DNA methylation predominantly occurred in regulatory enhancer regions, where known binding motifs of MRF, MEF2 and ETS proteins were identified. A transcriptional network analysis revealed modules harboring distinct ontologies, and interestingly the overall direction of the changes of methylation within each module was inversely correlated to expression changes. In conclusion, we show that highly consistent and associated modifications in methylation and expression, concordant with observed health-enhancing phenotypic adaptations, are induced by a physiological stimulus.
Overall design DNA samples from vastus lateralis muscle bisopsies were included in the study. Specifically, 23 subjects performed three months of supervised endurance training. Biospies were taken at rest, before and after training. DNA methylation levels were profiled using Illumina 450K arrays.
Contributor(s) Marabita F, Lindholm ME
Citation(s) 25484259
Submission date Aug 22, 2014
Last update date Mar 13, 2020
Contact name Francesco Marabita
Organization name Karolinska Institutet
Street address Karolinska University Hospital
City Stockholm
ZIP/Postal code 17176
Country Sweden
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (36)
GSM1484233 muscle_T1_1_rep1
GSM1484234 muscle_T2_1_rep1
GSM1484235 muscle_T1_4_rep1
BioProject PRJNA259207

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60655_RAW.tar 480.3 Mb (http)(custom) TAR (of IDAT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap