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Series GSE60603 Query DataSets for GSE60603
Status Public on May 06, 2015
Title An alternative pluripotent state confers interspecies chimaeric competency [RNA-Seq]
Organisms Macaca mulatta; Pan troglodytes; Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Here we show that by simple modulation of extrinsic signaling pathways, a new class of pluripotent stem cells, referred to as region selective epiblast stem cells (rsEpiSCs), could be efficiently derived from different stages of the early embryo. rsEpiSCs share features of primed pluripotency yet are distinct from EpiSCs in their molecular characteristics and ability to colonize post-implantation embryos. We performed RNA-sequencing experiments and examined the global gene expression profiles of EpiSCs, rsEpiSCs, in vivo isolated four regions of E6.5 mouse epiblasts: AP (anterior-proximal), AD (anterior-distal), PP (posterior-proximal) and PD (posterior-distal), human H1 ESCs, H1 rsESCs, H9 ESCs, H9 rsESCs, rhesus monkey ORMES23 rsESCs, and chimpanzee rsiPSCs.
Overall design Examination of global gene expression profiles in 2 pluripotent stem cell types across multiple species.
Contributor(s) Wu J, Benner C
Citation(s) 25945737
Submission date Aug 21, 2014
Last update date May 15, 2019
Contact name Christopher Benner
Organization name University of California, San Diego (UCSD)
Department Medicine
Street address 9500 Gilman Dr. MC 0640
City La Jolla
State/province California
ZIP/Postal code 92093-0640
Country USA
Platforms (4)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19129 Illumina HiSeq 2500 (Macaca mulatta)
Samples (14)
GSM1552685 EpiSCs Rep #1
GSM1552686 EpiSCs Rep #2
GSM1552687 rsEpiSCs Rep #1
This SubSeries is part of SuperSeries:
GSE60605 An alternative pluripotent state confers interspecies chimaeric competency
BioProject PRJNA258641
SRA SRP045673

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60603_fpkm.mouse-EpiSC.txt.gz 1.7 Mb (ftp)(http) TXT
GSE60603_fpkm.mouse-invivo.txt.gz 1.2 Mb (ftp)(http) TXT
GSE60603_fpkm.primates.txt.gz 1.3 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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