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Series GSE60520 Query DataSets for GSE60520
Status Public on Aug 19, 2017
Title Thymic epithelial cells induce formation of mesenchymal network structure after abrogation of thymic crosstalk
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In the earliest step of thymic organogenesis, mesenchymal cells support the growth of thymic epithelial cells (TECs). It is established that TECs and thymocytes influence each other for their growth/differentiation, a process called thymic crosstalk. However, little is known about the influence of developing thymocytes or TECs on mesenchymal cells. Here, we show that during normal thymus development fibroblast ingrowth occurs towards hypoxic areas. Similar overgrowth of mesenchymal cells is seen in a fetal thymic organ culture system under low oxygen conditions. However, when thymocytes were depleted by deoxyguanosine treatment, mesenchymal cells were also induced, precluding the direct effect of hypoxia. In the fetal thymus of hCD3εTg mice, which lack T lineage cells, an overgrowth of mesenchymal cells can be seen at a very early stage of thymic organogenesis. The growth of the mesenchymal cells is due to extensive proliferation and not mere enrichment. With RNA sequencing analysis comparing hCD3εTg with wild type TECs, we identified candidate factors that could be involved in mesenchymal network formation.
Overall design Comparison of thymic epithelial cells of different in vivo situations; one in presence of thymocytes, the other in the absence of thymocytes
Contributor(s) Satoh R, Kakugawa K, Kawamoto H
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Submission date Aug 19, 2014
Last update date May 15, 2019
Contact name Takaho A. Endo
Organization name RIKEN
Department IMS
Lab Laboratory for Integrative Genomics
Street address 1-7-22 Suehiro, Tsurumi
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (2)
GSM1481591 B6J
GSM1481592 humanCD3eTg
BioProject PRJNA258460
SRA SRP045606

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60520_RAW.tar 360.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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