|
| Status |
Public on Apr 23, 2015 |
| Title |
Dicer and Hsp104 function in a negative feedback loop to confer epigenetic robustness [RNA_profiles] |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Epigenetic mechanisms can be influenced by environmental cues and thus evoke phenotypic variation. This plasticity can be advantageous for adaption, but also detrimental if not under tight control. Although having attracted considerable interest, it remains largely unknown if and how environmental cues such as temperature trigger epigenetic alterations. Using fission yeast, we demonstrate that environmentally induced discontinuous phenotypic variation is buffered by a negative feedback loop that involves the RNase Dicer and the protein disaggregase Hsp104. In the absence of Hsp104, Dicer accumulates in cytoplasmic inclusions and heterochromatin becomes unstable at elevated temperatures, an epigenetic state that is inherited for many generations after the heat stress. Dicer instead averts the toxic aggregation of a prionogenic protein. Our results highlight the importance of feedback regulation in building epigenetic memory and uncover Hsp104 and Dicer as homeostatic controllers that buffer environmentally induced stochastic epigenetic variation and toxic aggregation of prionogenic proteins.
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| |
|
| Overall design |
wt_spb75 grown at 30°C or 37°C
|
| |
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| Contributor(s) |
Oberti D, Biasini A, Kirschmann MA, Jacobeit K, Genoud C, Stunnenberg R, Shimada Y, Bühler M |
| Citation(s) |
25543137 |
| |
| Submission date |
Aug 14, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Tim Roloff |
| E-mail(s) |
tim.roloff@fmi.ch
|
| Organization name |
FMI
|
| Department |
Functional Genomics
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL13988 |
Illumina HiSeq 2000 (Schizosaccharomyces pombe) |
|
| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE60640 |
Dicer and Hsp104 function in a negative feedback loop to confer epigenetic robustness |
|
| Relations |
| BioProject |
PRJNA258226 |
| SRA |
SRP045507 |
