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Series GSE60429 Query DataSets for GSE60429
Status Public on Apr 23, 2015
Title Dicer and Hsp104 function in a negative feedback loop to confer epigenetic robustness [RNA_profiles]
Organism Schizosaccharomyces pombe
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Epigenetic mechanisms can be influenced by environmental cues and thus evoke phenotypic variation. This plasticity can be advantageous for adaption, but also detrimental if not under tight control. Although having attracted considerable interest, it remains largely unknown if and how environmental cues such as temperature trigger epigenetic alterations. Using fission yeast, we demonstrate that environmentally induced discontinuous phenotypic variation is buffered by a negative feedback loop that involves the RNase Dicer and the protein disaggregase Hsp104. In the absence of Hsp104, Dicer accumulates in cytoplasmic inclusions and heterochromatin becomes unstable at elevated temperatures, an epigenetic state that is inherited for many generations after the heat stress. Dicer instead averts the toxic aggregation of a prionogenic protein. Our results highlight the importance of feedback regulation in building epigenetic memory and uncover Hsp104 and Dicer as homeostatic controllers that buffer environmentally induced stochastic epigenetic variation and toxic aggregation of prionogenic proteins.
Overall design wt_spb75 grown at 30°C or 37°C
Contributor(s) Oberti D, Biasini A, Kirschmann MA, Jacobeit K, Genoud C, Stunnenberg R, Shimada Y, Bühler M
Citation(s) 25543137
Submission date Aug 14, 2014
Last update date May 15, 2019
Contact name Tim Roloff
Organization name FMI
Department Functional Genomics
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
Platforms (1)
GPL13988 Illumina HiSeq 2000 (Schizosaccharomyces pombe)
Samples (3)
GSM1479698 wt_spb75_30C
GSM1479699 wt_spb75_37C_8h
GSM1479700 wt_spb75_37C_7d
This SubSeries is part of SuperSeries:
GSE60640 Dicer and Hsp104 function in a negative feedback loop to confer epigenetic robustness
BioProject PRJNA258226
SRA SRP045507

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Supplementary file Size Download File type/resource
GSE60429_RAW.tar 1.2 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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