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Series GSE60427 Query DataSets for GSE60427
Status Public on Jun 01, 2015
Title Toll-like receptor 10 in Helicobacter pylori infection
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Helicobacter pylori is a highly successful and important human pathogen that causes chronic gastritis, peptic ulcer diseases and gastric cancer. Innate immunity plays an important role of the primary defense against pathogens and epidemiological studies have suggested a role of toll-like receptor 1 (TLR1) in the risk of H. pylori acquisition. We performed microarray analysis of gastric mucosal biopsy specimens from H. pylori-positive and uninfected subjects; infection was associated with an ~15-fold up-regulation of TLR10 (p <0.001). Quantitative RT-PCR confirmed TLR10 mRNA levels were increased 3-fold in H. pylori-infection (p <0.001) and immunohistochemistory using anti-TLR10 polyclonal antibodies showed increased TLR10 expression in gastric epithelial cells of infected individuals. In vitro experiments where H. pylori was co-cultured with NCI-N87 gastric cells showed significant H. pylori-specific up-regulation of TLR10 mRNA levels and a correlation with TLR2 mRNA levels (R = 0.87, P <0.001). We compared combinations of TLRs for their ability to mediate NF-_B activation. NF-_B activation was increased following exposure to heat killed H. pylori or H. pylori-LPS only with the TLR2/TLR10 heterodimer. These findings suggest TLR10 is a functional receptor involved in the innate immune response to H. pylori infection and that TLR2/TLR10 heterodimer possibly functions in the recognition of H. pylori-LPS.
Overall design We have employed whole genome microarray expression profiling of gastric epithelium in H.pylori infection. We compare 1) Hp positive and negative group. 2) by gastritis grade 3) by countries.
Contributor(s) Nagashima H, Yamaoka Y
Citation(s) 25977263, 28739826
Submission date Aug 14, 2014
Last update date Jun 26, 2019
Contact name Hiroyuki Nagashima
Organization name Baylor College of Medicine
Department Department of Medicine Gastroenterology and Hepatology
Street address 2002 Holcombe Blvd. (3B-172)
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (32)
GSM1479654 Bhutan_normal_97
GSM1479655 Bhutan_normal_116
GSM1479656 Bhutan_normal_68
BioProject PRJNA258220

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60427_RAW.tar 397.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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