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Series GSE60328 Query DataSets for GSE60328
Status Public on Aug 01, 2020
Title Transcriptional profiling of CHB8 human embryonic stem cells with insulin receptor knockdown
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary While insulin receptor (IR) signaling is involved in the maintenance and proliferation of human embryonic stem cells (hESCs), its functional significance is poorly understood. To elucidate the role of IR in the pluripotency network, we knocked down INSR in CHB8 hESCs and performed RNA sequencing and differential expression analysis. We observe dysregulation of the AKT and ERK1/2 signalling pathways and downregulation of extracellular matrix genes.
Overall design RNA sequencing of CHB8 hESCs with shRNA knockdown of INSR.
Contributor(s) Teo AK, Mallard WJ, Gupta MK, Rinn JL, Gritsenko MA, Smith RD, Qian W, Kulkarni RN
Citation(s) 33667549
Submission date Aug 12, 2014
Last update date Apr 20, 2021
Contact name William Mallard
Organization name Harvard University
Department Stem Cell and Regenerative Biology
Lab Rinn Lab
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (4)
GSM1471692 shScr_rep1
GSM1471693 shScr_rep2
GSM1471694 shIR_rep1
BioProject PRJNA258044
SRA SRP045438

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Supplementary file Size Download File type/resource
GSE60328_gene_exp.diff.gz 1.7 Mb (ftp)(http) DIFF
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