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Series GSE6013 Query DataSets for GSE6013
Status Public on Mar 05, 2007
Title Gene expression in asbestos exposed lung cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Asbestos has been shown to cause chromosomal damage and DNA aberrations. The fiber is associated with many different lung diseases such as asbestosis, malignant mesothelioma, and lung cancer, but the disease-related processes are still largely unknown. Our aim was to identify specific gene expression profiles by using Affymetrix arrays, in human cell lines A549, Beas-2B, and MeT5A exposed to asbestos in a time-dependent manner. The hybridization data was analyzed using an algorithm specifically designed for clustering short time series expression data, a canonical correlation analysis (CCA) for identifying correlations between the cell lines, and a Gene Ontology (GO) analysis method for the identification of enriched differentially expressed biological processes.
Keywords: time course, gene expression, cell line, asbestos
 
Overall design Samples from three cell lines were collected from asbestos exposed and non-exposed controls at 1h, 6h, 24h, 48h and 7days (not all time-points for each cell lines). Replicates were done for one exposed sample of each cell line.
 
Contributor(s) Nymark P, Lindholm PM, Korpela MV, Lahti L, Ruosaari S, Kaski S, Hollmen J, Anttila S, Kinnula VL, Knuutila S
Citation(s) 17331233
Submission date Oct 11, 2006
Last update date Mar 25, 2019
Contact name Penny Nymark
E-mail(s) penny.nymark@ki.se
Organization name Karolinska Institutet
Department Institute of Environmental Medicine
Street address Nobels väg 13
City Solna
State/province Stockholm
ZIP/Postal code 17165
Country Sweden
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (27)
GSM139640 A549 asbestos exposed 1h
GSM139641 A549 asbestos exposed 6h
GSM139642 A549 asbestos exposed 7 days
Relations
BioProject PRJNA97813

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Supplementary file Size Download File type/resource
GSE6013_RAW.tar 149.2 Mb (http)(custom) TAR (of CEL)

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