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Series GSE59855 Query DataSets for GSE59855
Status Public on Jul 29, 2014
Title Gene expression profiling associated with knockdown of LKB1 in human intrahepatic cholangiocarcinoma
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The experiment was designed to display differential gene expression profiling in three human intrahepatic cholangiocarcinoma (ICC) cells upon knockdow of LKB1 tumor suppressor, by using RNAseq technology.
Overall design LKB1 was first attenuated in three ICC cells: HuH-28, RBE, and SSP-25 by siRNA-mediated knockdown. Total RNA was extracted from duplicated cancers cells transfected with control siRNA and LKB1 specific siRNA at 48h posttransfection for RNAseq analysis. Differentially expressed genes in LKB1-attenuated ICC cells were identified in comparison to that in ICC cells transfected with control siRNA.
Contributor(s) Zhang K, Yen Y, Wang J, Lu Y, Wu X
Citation(s) 26056085
Submission date Jul 28, 2014
Last update date May 15, 2019
Contact name keqiang zhang
Organization name City of Hope National Medical Center
Department Molecular Pharmacology
Street address 1500 East Duarte Road
City Duarte
State/province CA
ZIP/Postal code 91010
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (12)
GSM1447484 HuH28-Control-1
GSM1447485 HuH28-Control-2
GSM1447486 HuH28-LKB1-1
BioProject PRJNA256469
SRA SRP045048

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Supplementary file Size Download File type/resource
GSE59855_norm_RPKB_all.txt.gz 1.3 Mb (ftp)(http) TXT
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Processed data are available on Series record

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