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| Status |
Public on Aug 14, 2014 |
| Title |
Discovery of biomarkers predictive of GSI response in triple negative breast cancer and adenoid cystic carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Next generation sequencing was used to identify Notch mutations in a large collection of diverse solid tumors. NOTCH1 and NOTCH2 rearrangements leading to constitutive receptor activation were confined to triple negative breast cancers (TNBC, 6 of 66 tumors). TNBC cell lines with NOTCH1 rearrangements associated with high levels of activated NOTCH1 (N1-ICD) were sensitive to the gamma-secretase inhibitor (GSI) MRK-003, both alone and in combination with pacitaxel, in vitro and in vivo, whereas cell lines with NOTCH2 rearrangements were resistant to GSI. Immunohistochemical staining of N1-ICD in TNBC xenografts correlated with responsiveness, and expression levels of the direct Notch target gene HES4 correlated with outcome in TNBC patients. Activating NOTCH1 point mutations were also identified in other solid tumors, including adenoid cystic carcinoma (ACC). Notably, ACC primary tumor xenografts with activating NOTCH1 mutations and high N1-ICD levels were sensitive to GSI, whereas N1-ICD low tumors without NOTCH1 mutations were resistant.
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| Overall design |
Gene expression profiling for Notch-sensitive cancer cell lines using RNA-seq, each sample with triplicates
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| Contributor(s) |
Kremer B, Zang C, Taing L, Liu X, Aster JC, Pear WS |
| Citation(s) |
25104330 |
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| Submission date |
Jul 28, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Chongzhi Zang |
| Organization name |
University of Virginia
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| Street address |
P. O. Box 800717
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| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA256302 |
| SRA |
SRP044917 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE59810_CUTLL_GSI_v_DMSO.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
| GSE59810_CUTLL_GSI_v_WASH.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
| GSE59810_HCC1599_GSI_v_DMSO.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
| GSE59810_HCC1599_GSI_v_WASH.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
| GSE59810_REC1_GSI_v_DMSO.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
| GSE59810_REC1_GSI_v_WASH.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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