GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE59767 Query DataSets for GSE59767
Status Public on Jul 20, 2015
Title N6-adenosine methylation in miRNAs
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Methylation of N6-adenosine (me6A) has been observed in rRNA, tRNA, snoRNA, lncRNA and mRNA (ref.), but not yet miRNA. Interestingly, many mRNAs contain miRNA-binding sites and me6A in their 3'-UTR, although they do not appear to overlap (Mayer). We have investigated whether adenosine methylation affects miRNA levels and whether miRNAs are methylated. Indeed, we have obtained evidence suggesting that miRNA can be methylated and that the steady state level of certain miRNAs is affected by the level of the 6meA demethylase FTO.
Overall design RNA-Seq of small RNAs prepared from FTO knockdown cells. RNA-Seq of small RNA fraction first immunoprecipitated by antibody against me6A
Contributor(s) Berulava T, Rademacher K, Klein-Hitpass L, Rahmann S, Horsthemke B
Citation(s) 25723394
Submission date Jul 25, 2014
Last update date May 15, 2019
Contact name Ludger Klein-Hitpass
Phone +49 201 723 85552
Organization name Institut fuer Zellbiologie
Department Universitaetsklinikum
Lab BioChip Lab
Street address Virchowstr. 173
City Essen
ZIP/Postal code D-45122
Country Germany
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (15)
GSM1446243 FTO1C1_scr
GSM1446244 FTO1C1_FTO kd
GSM1446245 FTO2D4_scr
BioProject PRJNA256175
SRA SRP044847

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59767_RIP-Seq_read_counts.txt.gz 31.9 Kb (ftp)(http) TXT
GSE59767_RNA-Seq_FTO_kd_read_counts.txt.gz 28.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap