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Series GSE59717 Query DataSets for GSE59717
Status Public on May 26, 2015
Title Wide-spread disruption of transcription termination in HSV-1 infection: Next generation sequencing of total and newly transcribed (4sU-RNA) RNA
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Primary human foreskin fibroblasts (HFF) were infected with wild-type simplex virus 1 (HSV-1) strain 17 at a multiplicity of infection (MOI) of 10. Newly transcribed RNA was labelled by adding 500µM 4-thiouridine (4sU) to the cell culture media for 1h. Total cellular RNA was isolated using Trizol. Newly transcribed RNA was purified following the protocol described in Raedle et al. JoVE 2013.
Overall design Newly transcribed RNA was labelled in one hour intervals during the first eight hours of HSV-1 infection. All nine 4sU-RNA samples as well as total cellular RNA of every second hour of infection were sent for sequencing. Two independent biological replicates were analysed.
Contributor(s) Rutkowski A, Erhard F, L'Hernault A, Bonfert T, Schilhabel M, Crump C, Rosenstiel P, Efstathiou S, Zimmer R, Friedel CC, Dölken L
Citation(s) 25989971, 29579120, 28135292
Submission date Jul 23, 2014
Last update date Jul 10, 2019
Contact name Caroline Friedel
Organization name Ludwig-Maximilians-Universität München
Department Institut für Informatik
Street address Amalienstr. 17
City München
ZIP/Postal code 80333
Country Germany
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (28)
GSM1444166 Total RNA - mock Rep 1
GSM1444167 Total RNA - 2hpi Rep 1
GSM1444168 Total RNA - 4hpi Rep 1
BioProject PRJNA256013
SRA SRP044766

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Supplementary file Size Download File type/resource
GSE59717_RAW.tar 11.1 Mb (http)(custom) TAR (of RPKM)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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