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Series GSE59615 Query DataSets for GSE59615
Status Public on Nov 15, 2014
Title Transcriptional and epigenetic program changes of induced beta cells over time [RRBS-seq]
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary Direct lineage conversion is a promising approach to generate therapeutically important cell types for disease modeling and tissue repair. However, it is often unclear whether lineage-reprogrammed cells remain stable long-term and whether the properties of the reprogrammed cells evolve over time. Here, using an improved method of converting pancreatic acinar cells to beta-cells, we show that induced beta-cells persist in the adult pancreas for up to 14 months and form islet-like structures. Detailed analyses of induced cells over 7 months reveal that global DNA methylation changes occur rapidly whereas transcription network remodeling evolves over two months to resemble that of endogenous beta-cells and then stabilizes thereafter. Progressive gain of beta-cell function by converted cells during the 7 month period coincides with both transcriptional changes and the formation of islet-like structures. These studies demonstrate the ability of lineage-reprogrammed cells to achieve a stable state and identify key cellular and molecular milestones during their long-term evolution.
Overall design Acinar cells and beta cells were collected as control, as well as induced beta cell samples at day 10, day 30, day 60, and 7 months
Contributor(s) Li W, Zhu J, Zhang Y, Clement K, Zhou Q
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jul 21, 2014
Last update date May 15, 2019
Contact name Kendell Clement
Organization name Harvard University
Lab Meissner Lab
Street address 7 Divinity Ave.
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM1440536 Acinar
GSM1440537 Induced beta cell d10
GSM1440538 Induced beta cell d30
BioProject PRJNA255762
SRA SRP044672

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Supplementary file Size Download File type/resource
GSE59615_RAW.tar 66.9 Mb (http)(custom) TAR (of BED)
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Raw data are available in SRA
Processed data provided as supplementary file

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