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Series GSE59498 Query DataSets for GSE59498
Status Public on Jun 01, 2022
Title Synthetic amorphous magnesium-substituted calcium phosphate: silent constructs that trap macromolecules for intracellular delivery
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Amorphous magnesium-substituted calcium phosphate (AMCP) nanoparticles form constitutively in large numbers in the gastrointestinal tract. Collective evidence indicates that they trap luminal macromolecules, such as bacterial and dietary protein antigen, delivering this cargo to mucosal antigen presenting cells. Here using a precise synthetic mimetic of the AMCP nanomineral, we studied whether trapping of macromolecules by AMCP modulates signalling of the macromolecule itself. Based upon whole genome transcriptomic analyses and using peptidoglycan as a macromolecule, we showed that neither AMCP nanomineral itself regulated any gene, nor did it modify any gene regulation by peptidoglycan. We conclude that synthetic AMCP nanomineral can deliver macromolecular cargo intracellularly without affecting gene transcription.
Overall design Peripheral blood mononuclear cells (PBMC) were isolated from 7 subjects and further enriched for monocytes by Percoll density gradient centrifugation. Protein-containing (bovine serum albumin at 500 µg/ml), synthetic amorphous magnesium-substituted calcium phosphate nanoparticles were prepared in the absence (AMCP) or presence (AMCP/sPg) of 50 µg/ml soluble, purified peptidoglycan from Escherichia coli. Monocyte-enriched PBMC were stimulated for 3 hr with synthetic AMCP or AMCP/sPg nanoparticles (both diluted 1:9 in tissue culture medium) or with 4 µg/ml soluble peptidoglycan (sPg) or with vehicle control. Cells were subsequently recovered and subjected to genome-wide gene expression analysis.
Contributor(s) Haas CT, Boekschoten MV, Pele LC, Powell JJ
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Submission date Jul 16, 2014
Last update date Jun 02, 2022
Contact name Guido Hooiveld
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (28)
GSM1438435 Mono-enriched-PBMC_ctrl_3hr_subj1
GSM1438436 Mono-enriched-PBMC_ctrl_3hr_subj2
GSM1438437 Mono-enriched-PBMC_ctrl_3hr_subj3
BioProject PRJNA255435

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Supplementary file Size Download File type/resource
GSE59498_RAW.tar 122.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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