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Series GSE59484 Query DataSets for GSE59484
Status Public on Jul 17, 2014
Title Expression data for miR-33a over-expression in CD133-negative D456MG cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary MiR-33a is involved in the maintenance of Glioma Initiating Cells (GIC) and tumor progression. MicroRNA-33a could promote GIC growth and self-renewal by regulating two pathways including cAMP/PKA pathway and Notch pathway. We used microarrays to identify the direct target genes of miR-33a in a glioblastoma cell line D456MG.
We used microarrays to detail the global change of gene expression after miR-33a over-expression and identified target genes during this process.
Overall design Cells were infected with lenti-virus expressing a control vector (pWPXLD) or human primary miR-33a. Then RNA was extracted and gene expression was profiled by Affymetrix microarray.
Contributor(s) Wang H, Sun T, Hu J
Citation(s) 25202981
Submission date Jul 16, 2014
Last update date Dec 06, 2018
Contact name Hui Wang
Organization name Duke University
Street address 380 Research Drive
City Durham
State/province NC
ZIP/Postal code 27707
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (2)
GSM1437722 CD133-negative D456MG cells infected with vector control
GSM1437723 CD133-negative D456MG cells with miR-33a over-expression
BioProject PRJNA255417

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Supplementary file Size Download File type/resource
GSE59484_RAW.tar 4.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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