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Series GSE59481 Query DataSets for GSE59481
Status Public on Mar 20, 2015
Title Ptf1a promotes an inhibitory peptidergic identity in cortical pyramidal cells in vivo
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Ptf1a has been shown to be necessary for determining an inhibitory interneuronal fate in many regions of the nervous system. In this study, we aim to investigate the sufficiency of Ptf1a to cell-autonomously promote a specific neuronal identity by misexpressing it in developing excitatory cortical pyramidal cells and studying its impact on pyramidal cell features, such as its gene expression profile. To accomplish this, we electroporate either a Ptf1a/GFP-misexpression construct or a GFP-only control construct into E12.5 mouse embryonic cortices, harvest the cortices at E15.5, and examine Ptf1a-induced changes to the pyramidal cell transcriptome, molecular expression pattern, neurotransmitter status, and morphology. We conclude that Ptf1a is sufficient to cell-autonomously promote an inhibitory peptidergic identity and alter neuronal morphology in developing cortical pyramidal cells. The results of this study provide insight into intrinsic transcriptional controls over neuronal identity, specifically implicate Ptf1a as a potent regulator of an inhibitory peptidergic identity, and may guide future studies of neuronal reprogramming for circuit repair after disease or injury.
Overall design Purified GFP-expressing cells were isolated from mouse embryonic cortices electroporated with either a Ptf1a/GFP-misexpressing construct or a GFP-only control construct. 3-6 brains were collected per sample, yielding 58-106,000 cells per sample. Ptf1a/GFP-misexpressing samples were run in triplicate, GFP-only control samples were run in duplicate.
Contributor(s) Russ JB, Borromeo MD, Kollipara RK, Bommareddy P, Johnson JE, Kaltschmidt JA
Citation(s) 25878276
Submission date Jul 16, 2014
Last update date May 15, 2019
Contact name Julia Kaltschmidt
Organization name Sloan-Kettering Institute
Street address 1275 York Av
City New york
ZIP/Postal code 10065
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (5)
GSM1437638 GFP-only control sample 1
GSM1437639 GFP-only control sample 2
GSM1437640 Ptf1a-misexpression sample 1
BioProject PRJNA255411
SRA SRP044368

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Supplementary file Size Download File type/resource
GSE59481_PIG_vs_GFP_DE_genes.txt.gz 787.9 Kb (ftp)(http) TXT
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